# Introduction olcanic regions have always attracted many people worldwide because of the high fertility of their soils (Diana et al., 2019). However, human proximity to volcanoes can lead to several health problems as consequence of the chronic exposure to the materials released from the volcanic activity. An element often found in elevated concentrations in volcanic regions is fluorine. Although fluoride is recognized to have a beneficial effect on the rate of occurrence of dental caries when ingested in small amounts, its excessive intake results in a widespread but preventable pathological disease called fluorosis (Dey and Giri, 2015) While skeletal fluorosis, the most severe form of fluorosis, requires a chronic exposure to high concentrations of fluoride in water (4-8 mg/L), dental fluorosis occurs after shorter periods of exposure to fluoride in lower concentrations (1.5-2.0 mg/L). In some volcanic regions, where exposure to elevated amounts of fluoride is persistent, biomonitoring programs are fundamental (Garcia and Borgnino, 2015). In the present world pollution scenario, a comprehensive knowledge of pollutants and their adverse effects on the ecosystem are required for selection of a workable monitoring and conservation technique (Munzi et al., 2012;Ahmad et al., 2007). The increasing awareness of the potential hazards and impact of air pollution on the health of human populations, forest decline, climate change and loss of agricultural productivity, for example, has been a cause of increasing public concern throughout the world (Smodis, 2007). This has highlighted the need for continuous monitoring of the levels of pollutants in the environment (Garty et al., 2002). Environmental monitoring approaches that are cheap, can be used anywhere, and respond to many kinds of airborne pollutants are needed to fingerprint the pollutant sources and their dispersion pattern (Larsen et al., 2007). A comprehensive approach to reduce the impacts of pollution and climate change, an approach that decreases emissions across all sectors and enhances the adaptive capacity of all nations with economic reflections is needed (Pinhoa et al., 2012). Lichens emerge as the key answer to this monitoring problem and are the flora of choice for monitoring studies (Notcutt and Davis, 1989). They obtain their nutrients directly from the atmosphere and their chemical composition therefore holds the promise of becoming a natural or 'green' technique for monitoring the health of the environment around passively degassing volcanoes and industries. Bioaccumulation in lichen thalli has been used as a major tool for assessing air quality in volcanic and industrial areas (Bennett, 2006). They are extremely valuable in environmental monitoring since they exist worldwide and are sensitive to many different kinds of pollutants (Brodeková et al., 2006). They are slow growing; do not shed parts and are perennial pioneer plants commonly described as sentinel organisms (Loppi et al., 2002). They are good bio-accumulators of heavy metals and trace elements and can be transplanted where they do not occur in nature (Llop et al., 2012). Lichens are mutual symbiosis between fungi with an algal and/or a cyanobacterial partner (Morris and Purvis, 2007). The success in lichenization is attributed to a genetic combination resulting from metabolic biomolecules and influenced by environmental factors (Jatinder et al., 2012). This process has created unique characteristics in lichens such as the unique anatomical (absence of roots, stomata, vascular tissues and cuticle) and physiological (poikilohydry and absorbance of nutrient from general thallus surface from the atmosphere. These peculiarities, allow lichens to grow in all sorts of terrestrial habitats comprising 8% of vegetation. According to Lawrey (1986), lichens produce a wide array of more than 1000 unique secondary metabolites (depsides, depsidones, ?-orcinoldibenzyl esters, and xanthones, usnic acid and pulvinic acid derivatives, for example) as adaptations for life in marginal habitats. These secondary metabolites assist to maintain the lichen symbiotic association and compete with organisms sharing the same niche (Culberson and Culberson, 2001). Another characteristic stress-resistance mechanism is the accumulation of melanin and oxalate crystals in their thallus, which provide a crystal layer on the thallial surface making lichens tolerant to extreme environments and good bioaccumulators of atmospheric substances (Hess et al., 2008). Most lichens are tolerant to high concentrations of atmospheric pollutants well beyond levels necessary for their physiological requirement by sequestrating and accumulating varied oxalate crystals (Garty et al., 2002;Bjerke et al., 2002;Chen et al., 2000). The aggregates of these oxalate crystals disintegrate and provide a crystal layer on the thallial surface making lichens good bioaccumulators. Since lichens do not shed parts (Walker et al., 2003;Monge-Nájera et al., 2002), and bioaccumulate pollutants safely in their thalli over time, pollutants levels can be professionally determined quantitatively by chemical analysis of species and qualitatively by observing species diversity, abundance and distribution (Jovans, 2008). With their indiscriminate ability to absorb and bio-accumulate both nutrients/ pollutants from the atmosphere, elevated concentrations of certain elements in lichens are a sure sign of atmospheric deposition (van Herk, 1999). Lichens can be used as bio-monitors of pollutants by quantifying the amount of trace element(s) accumulated within them over time (Srivastava et al., 2015, Bargagli, 2016). They have been used to assess deposition and air quality in hundreds of studies worldwide (Donahue, 2018). Ayrault et al., 2007, have shown a relationship between the quantities of pollutants in the environment and those concentrated in lichen thallus. A variety of elements and chemical compounds affecting lichen growth and distribution are found in the atmosphere (Bajpai et al. 2011). Pollutants, including sulfur dioxide (SO 2 ), nitrogen dioxide (NO 2 ) and fluoride (F) compounds, remain in the same chemical form after they are emitted into the atmosphere and are easily absorbed by lichens. Gases like chlorine and fluorine, leads to the injury of fundamental metabolic processes, which arise by acidifying the water and the substrates, resulting in the loss of most sensitive lichen species (Brodeková et al., 2006). Many lichens are sensitive to fluorine pollutant as it can concentrate in hydrated lichens to more than 200 times ambient concentrations (Notcutt and Davis, 1989). Fluoride are highly toxic to lichens, and elevated levels of fluoride are correlated with chlorophyll breakdown, reduced ATP concentration, reduced photosynthesis and disappearance of species (Stefano and Luisa, 2006). In general, obvious damage to lichens begins at levels of 50-70 ppm. In most parts of Europe like Germany for example, (Hauck, 2005), lichen transplants from pristine to polluted areas are carried out to bioaccumulate atmospheric pollutants. The lichen Hypogimnia physodes was used to bio-accumulate radionuclide Uranium (Golubev et al., 2006) and rare earth elements in Czech Republic (Jitka et al., 2010). The fruticose lichen Stereocaulon vesuvianum, growing on the slopes of Mount Vesuvius in South Italy, was used as a bio-monitor of 134 Cs, 137 Cs, 103 Ru and 106 Ru derived from the April 26, 1986 Chernobyl nuclear reactor disaster (Environment Canada, 2003). Grasso et al. (1999) found that lichen composition reflects the contribution of the volcanic activity in Mount Etna and Vulcano Island. They noted that, distribution of the degassing elements (arsenic (As), antimony (Sb), Br, and lead. Volcanoes emit a variety of gases both between and during eruptions, including H 2 O, CO 2 , SO 2 , HCl, NH 3 , H 2 S, HF and a few other minor constituents (Cronin and Sharp, 2002). These gases interact rapidly with the ash particles of a volcanic plume and especially atmospheric water to form acidic aerosols. These aerosols given off during and after volcanic eruptions caused problems on a number of occasions when it has accumulated in low lying areas. Exposure to excessive amounts of fluoride may cause adverse health effects for humans and animals (Conti et al., 2016) The plume dispersed by winds after a volcanic eruption contains volcanic ash that may also be a source of fluoride at levels that are potentially toxic. Fluorides are released into the environment naturally through the weathering and dissolution of minerals, the emissions from volcanoes and from marine aerosols (WHO, 2002) fluoride emissions from volcanoes and the natural occurrence of excessive amounts of fluoride in drinking water have affected the health of humans and livestock for centuries, if not millennia. Although sometimes of anthropogenic origin, high levels of fluorine are generally related to natural sources. Volcanic emissions of fluorine take the form of either sluggish permanent release from quiescent volcanoes (passive degassing) or rarer but more impacting discharges during short-lived volcanic eruptions (Schwandner et al., 2004Linhares et al., 2017). It has been estimated that passive degassing, like that existing at Mt. Etna (Italy) and Masaya (Nicaragua) volcanoes, accounts for about 90% of the volcanic fluorine release. The influence of these emissions on the surrounding environment and in particular on vegetation has been investigated by several authors (Nelson and Wheeler, 2016). Little or no bio-accumulation and monitoring work have been carried out on the active Mt. Cameroon. Mount Cameroon volcano with a return period of 20 years (Suh et al., 2003), has been the most frequent erupted volcano in West Africa, with eight eruptions in the last 100 years (1909, 1922, 1925, 1954, 1959, 1982, 1999 and 2000). It constantly releases various constituents into the environment during active eruptions and even in quiescent degassing periods (Suh et al., 2008). These researchers reported that, Mt. Cameroon basanites melt inclusions has shown high levels of carbon dioxide with a concentration of 967 µg/g, sulphur 2400 µg/g, chlorine 1270 µg/g and fluorine 1530 µg/g. In spite of these findings, there is little knowledge of lichens toxic levels and remediation of the high levels of halides release from this volcano. The objective of this paper was to determine the concentration levels of some halogens and identify potential lichens species that can be used as appropriate bio-accumulators of halide toxicity for Mt. Cameroon degassing volcano. # II. # Materials and Methods # a) Description of the Study Area i. Location The study area (Fig. 1) is the active MC volcano located in the coastal belt of the Gulf of Guinea, South West Region of Cameroon. It lies between Latitudes 3 º 57' to 4 º 27'N and Longitudes 8 º 58' to 9 º 24'E (Suh et al., 2003). It is the highest peak in West Africa, is of volcanic origin and rises from the Atlantic Ocean to a height of 4100 m. It covers a surface area of about 1750 km 2 (DeLancey and Mark, 2000). The survey sites were divided into Northern and Southern contrasting flanks following wind direction and ash fall trends from various eruptions. The northern flank was called the leeward and southern flank was called the windward. Out of the eight sites selected on the two flanks, four were on the leeward flank (Lower Buea, Upper Buea, IRAD-Ekona and Ekona-Mbenge) and four from the windward flank (Batoke, Bakingili, Idenau and Bomana). Lower Buea on the leeward flank comprises of University of Buea campus, Great Soppo, and Sasse. Some species were collected from control area of Mamfe (5.7512 º N -9.3146 º E) about 270 km from Mt. Cameroon. The survey was also done based on altitudinal levels which ranged between 3 to 2178 m above sea level (Table 1). The altitudinal levels were divided into three (low, mid and high) ranges. # c) Sample Selection Thirty-four macro lichens (Foliose and Fruticose) species from six families, eight genera were collected from 8 sites around Mt. Cameroon. From each sites, different sampling points were surveyed given a total of about 12 sampling points in the study. These species were collected from trees and rocks (Table S2). All the collecting points were Georeferenced with a High Sensitive ErexGlobal Positioning System (GPS). The samples were selected based on the criteria shown on Table 2. The selected species were common to most sites and represent various altitudinal levels on the two flanks of the edifice. # d) Sample preparation and analytical procedure In the Life Sciences laboratory, University of Buea, the lichen species to be analysed for their halogens (F, Br and Cl) levels, were sorted and curated to remove adhering bark, mosses, other lichen species, soil particles, etc. Following Lorenzini et al., 2006, no washing procedure was done, to avoid the leaching of soluble matter from tissues. The species were put in labeled envelops and oven-dried to constant weight in a Gallenkemp Hot box oven fan size 3 at 60 º C for 48 hours. The different species were put in small zip locked bags and labeled with chemical codes. Samples were chemically analysed by selective ion electrode method, at the department of geology, university of Botswana. A 0.5 g split of each sample was digested with hydrogen fluoride (HF), then aqua regia and the aliquots analysed. The detection limits ranged from 0.01 to 0.02 ?g/g. Replicate analyses were performed on selected samples and data quality was excellent with standard deviation values less than 1%. Standards were run between samples and quality control of the analyses was ensured by inserting blanks into the analytical run after 6 samples. Prior to statistical analysis of the geochemical parameters, the data set was regrouped based on the lichen species. The entire data were then log-transformed to normalise skewed distributions. The significance of the difference between means was tested using ANOVA test to compare the concentration of Halogens according to Elevation, Post Hoc Multiple Comparisons Altitudes, Independent Samples t-Test to compare the concentration of Halogens according to substrate types and Box -plot to confirm the test. Volume XXII Issue IV Version I # Results ANOVA test on the variation in the concentration of halogens across the different elevation revealed that there was a significant difference (p = 0.022) for F and Br and p= 0.030 for Cl at 95% confidence level (Table 2 Student t-test for the comparism of the halogens with regards to the substrate (Table 4) shows no significance difference in the means of the all the halogens but a slight difference (p= 0.048 at 0.05 levels) in the variance of Cl. # B Table S2 revealed that, of the eight lichen genera used for analyses, Leptogium was the most abundant, widely distributed and had very high concentrations of halogens compared to all the other species. Leptogium gelatinosum accumulating ability differs between sites. Leptogium gelatinosum samples from northern leeward flank (MC 02 from Upper Buea, MC 04 from Bomana and MC 10 and MC 11 from Ekona-Mbenge) show low accumulation of halogens over samples collected from the western windward flank Sasse (MC01 and MC03), Idenau (MC 05 and MC06), Bakingili (MC07) and Batoke (MC08 and MC09). Although, Leptogium gelatinosum recorded highest concentrations, other species like Heterodermia obscurata, Cladonia sp, Usnea articulate in BmtUM recorded high concentrations. Lichen species also showed differing accumulating potentials in substrates (Parmotrema tinctorum (MC27) a corticolous sample collected in Ekona-Mbenge accumulated more than the saxicolous specimen (MC26) collected in the same point). All the eight lichen genera showed very high concentrations of F and low levels of Br. F concentration is highest in Leptogium (188 ?g/g) and lowest in Parmotrema (0 ?g/gto 23 ?g/g). The geochemical analysis of the lichens indicated that F was the most dominant halogen (Fig. S1) with concentrations that range from 25-188 (mean = 78.00 ± 48.68 ?g/g). The range for Cl concentration was 10-127 ?g/g (mean =47.23 ± 33.37 ?g/g), while that for Br, ranges from 0-63 ?g/g (mean= 18 ± 19.58). Fig. S2 shows that, there was highest F-bioaccumulation in Leptogium gelatinosum species in Idenau which ranges from (153 to188 ?g/g), Batoke (165 ?g/g), Bakingili (178 ?g/g), Sasse (152 to155 ?g/g) and low in Ekona-Mbenge (34 ?g/g). Cl bio-accumulation in the Leptogium gelatinosum species in Idenau ranges from (110 to119 ?g/g) was highest and lowest in the Leptogium gelatinosum of Bomana (23 ?g/g), Ekona (33 ?g/g) and Hut 1(28 ?g/g) (Fig. S3). Fig. S4, revealed no Br bio-accumulation in the Leptogium gelatinosum species in Ekona (0 ?g/g) and Bomana (0 ?g/g) but higher in Idenau (63 ?g/g). # IV. Discussion Lichens from Mt. Cameroon demonstrated significant compositional variation between species as observed on the multi-element distribution patterns even for those growing in the same area. However, specimens from the same species tend to have similar element concentration patterns. This could be explained by the fact that lichens species selectively accumulated some elements. Similarly, Rani et al. (2011) found out that, the estimated nine heavy metals in lichen samples from 12 different sites of Dehradun city by periodic monitoring and spotted Zn, Ni, Cd and Cr were higher in lichens, collected from road side while maximum quantity of Fe, Cu and Al were reported in lichens collected from central sites of the city while the lowest amounts of all the metals were reported in sites farther from city. The species Leptogium gelatinosum with very high concentrations of the halogens in all the sampling sites and even the control area of Mamfe 270 km from Mt. Cameroon has a higher tendency to sequestrate these elements than all the other species recorded in the study. This is in accordance with the study about the suitability of the fruticose lichens Evernia prunastri, Cetraria islandica and Ramalina farinacea collected from oak trees in a remote area located in the Chianti Region (Tuscany,central Italy), as transplants for biomonitoring trace element, showed that E. prunastri has to be preferred for its higher accumulating capacity (Cercasov et al., 2002). Different lichens species in different environments have different sequestration potentials, for example, Ramalina fastigiata has been used as a bio indicator of the impact of a coal mine in Portugal Jó?wiak, (2012). The very high concentrations of F in lichens in this study reflects the study of Suh et al. (2008) who measured halogen content in melt inclusions at this volcano and their results indicated an average value of 1530 ?g/g F and 1270 ?g/g Cl. They recorded unusually high F concentrations when compared to glass inclusions from Etna, Hawaii and Piton de la Fournaise. However, the Cl concentrations from Mt. Cameroon were midway between the high values measured for Etna and the low values for Hawaii and Piton de la Fournaise. These exceptionally high values relative to those recorded in this study which maybe an indication that these halogens are an important component of the volatile budget at Mt. Cameroon. These researchers reported that, the concentrations of F in olivine hosted glass inclusion from Mt. Cameroon are the highest known F concentrations for basaltic glass inclusions in the world. Altitude contributed to the halogens concentration variations as intra-species variations consistently yield high concentrations in samples collected from the downwind SW flank of the volcano. These localities lie in the path of wind bearing volcanic gas plumes from Mt. Cameroon and therefore pin their higher halogen content to passive degassing. Aiuppa et al. (2004) reported that, during explosive activity huge quantities of fluorine are deposited with ashes around the volcano up to distances of hundreds of km. Fluorine is present as an adsorbed outer layer on the tephra particles which adsorption occurs by condensation of fluoride onto the tephra particles in the plume above the volcano as it cools. The smaller tephra particles have a larger surface area, so carry more absorbed fluoride to be carried further from the volcanic source, and so their greater fluorine-carrying capacity extends the zone of potential fluorine poisoning considerably, even to regions where only a 1 mm thick deposit forms. It is advisable to sample and analyse the tephra or vegetation to identify hazardous regions. This suggests that gases emitted from the volcano are blown south-westwards and are eventually deposited close to the coast resulting in higher halogen content in the lichen species from these areas. More so, the inputs from the sea and from agricultural farms might have increased the high levels in this coastal areas. Studies by Ndlovu et al. (2019), on moss and lichen biomonitoring of atmospheric pollution in the Western Cape Province (South Africa) observed halogens to have elevated concentrations for samples collected from areas with close proximity to the ocean. That is, for both moss and lichen samples at areas closer to the ocean had higher halogen concentrations. Their results also confirmed elevated concentration levels for halogens (Cl, I, Br) in areas closest to the ocean. However, since fluorides are released into the atmosphere in large amounts by volcanic eruptions (Bajpai et al., 2011) and fluoride (F) compounds, remain in the same chemical form after they are emitted into the atmosphere, the very high levels of F bioaccumulation in this study, might have come from the degassing volcanic winds of Mt. Cameroon. Also, since other sampling points inland shows high concentration of F, degassing winds and ash deposition should have a greater influence. In this study, even though Leptogium gelatinosum was the highest bioaccmulator, Stictastenroos and Heretodemia obscurata are also good accumulators, while all the Usnea species are poor accumulators. According to Brodo et al. (2001) fruticose lichens like Usnea are very sensitive to air pollutants than foliose lichens and occur only in very pure environments. Out of different growth forms of lichens, foliose lichens are prior to metal accumulation followed by crustose and squamulose lichens (Kar et al., 2014) and least by fruticose lichens (Shukla et al.,2014). However, lichens from the Usnea species have been used to evaluate heavy metal deposition patterns in the Antarctic (Poblet et al., 2011). Certain epiphytic lichens have been particularly gained attention for their bioaccumulation potential like Hypogymnia physodes for bioaccumulation of trace elements and Pyxine cocoes for bioaccumulation of metals (Bajpai et al., 2012;Daimari et al., 2020). The differing accumulating potentials in substrates of lichen species in this study, (example, Parmotrema tinctorum (MC27) a corticolous sample collected in Ekona-Mbenge accumulated more than the saxicolous specimen (MC26) collected in the same point).Contrary, the findings of (Chettri et al.,1997), who used the lichen species Neophuscelia pulla and Xanthoparmelia taractica to study the bioaccumulation of heavy metals in abandoned copper mines in Greece, where there was a significant correlation between the copper content in the soil(saxicolose) and that of the tree(corticolous) lichen thalli. However, it is for this reason that most studies use epiphytic macro lichens as bio-monitors for air pollutants (Loppi and Pirintsos, 2003). For example, Käffer et al. (2011) also reported corticolous lichens as environmental indicators in urban areas in southern Brazil. Furthermore, the no to slight significant difference in means of halogens concentrations with regards to substrates in this study is in accordance with the study of Bajpai et al. (2011) in Mandav city in central India illustrated that although most of the metals were absent, or present in insignificant amount in substrates, yet the thallus of lichens had significantly higher concentration of metals such as Cd, Cr, Ni and Zn. Thus it is apparent that the accumulated metals were air borne. All the eight lichen genera showed very high concentrations of F and low levels of Br. Weinstein et al. (1998) reported that, Br and I emissions are not usually of environmental importance and there is virtually no scientific literature on either element. The gas Cl is potentially very hazardous but it is very rare to be released in sufficient quantities to pose a risk (Temple et al., 1998). Chlorine concentrations of 0.4 -2.5 ?g/g range cause severe symptoms like upper surface bleaching, epinasty (distorted growth), chlorosis (yellowing) and leaf drop to plants (Temple and Krause, 1998). The Cl/F ratio in the specimens' ranges from 0.29 to 0.94, which is lower than those measured in lichen specimens at Mt. Etna which ranges from 0.51 to 1.46 (Notcutt and Davies, 1989). According to Delmelle et al. (1997) changes in the Cl/F ratio may reflect different physico-chemical behaviour of the gases entering the atmosphere. However, Halmer et al. (2002) reported that in areas without nearby emission sources, the mean concentrations of fluoride in ambient air are generally less than 0.1µg/g. This was observed from the control area (Mamfe 270 Km) with lower concentrations as compared to those from Mt. Cameroon. Even near emission sources, the levels of airborne fluoride usually do not exceed 2-3 µg/g and in most soils, fluoride is present at concentrations ranging from 20 to 1000 µg/g. This figure can reach several thousand µg/g in mineral soils with natural phosphate or fluoride deposits. Therefore, the atmospheric halogens load at Mt. Cameroon is significantly high and lichens can be potential monitors of this volcanic gas flux. V. # Conclusion Leptogium gelatinosum and Heretodemia obscurataare good accumulators, while Usnea species poor accumulators and therefore can be used for pollution bio-monitoring programs in Cameroon. The Leptogium gelatinosum species is therefore a suitable species for monitoring passive degassing at Mt. Cameroon. Also, considering that, lichens of the windward flank of MC accumulated more elemental contents than those from the leeward flanks, shows that wind direction and ash fall contribute largely to pollutant load in lichen species in the windward flanks of mount Cameroon reflecting volcanic degassing as the source. This chemical analysis serves as a baseline data for future studies. Volume XXII Issue IV Version I 1![Fig. 1: Topographic features of Mt. Cameroon and location of sampling sites mentioned in the text.](image-2.png "Fig. 1 :") 3![Fig. 3: Box -plot of Halogens concentrations in the different elevations The Post Hoc Multiple Comparison test (Table3) shows a significance difference at 0.05 levels of the halogens at low -mid altitude, F (p=0.030), Cl (p=0.027) and no significance difference of Br (p=0.053) at this altitude.](image-3.png "Fig. 3 :") ![The box-plot (fig 3) also confirms the slight difference in Cl. Volume XXII Issue IV Version I 24 ( )](image-4.png "") 3![Fig. 3: Box -plot of Halogens concentrations in the substrate types](image-5.png "Fig. 3 :") ![Lichen Species as Bio-Accumulator of Some Halogens on Mount Cameroon Volcano, West Africa Supplementary Material](image-6.png "B") S1![Figure S1: The different Halide concentrations in lichen species studied at the various sampling sites.](image-7.png "Figure S1 :BB") S2![Figure S2: F bio-accumulation in Leptogium gelatinosum from different sampling sites on MC.](image-8.png "Figure S2 :") 1AltitudeRange(m)SitesLow3 -499IRAD-EkonaBatokeBakingiliIdenauMid500-1000Ekona-MbengeLower BueaBomanaHigh>1000Upper Buea 2S/N Criteria (species abundance at sites, elevation, flanks, morphology)Species1.Foliose Species common to all sampling sitesLeptogium gelatinosum,2.Species of mid elevationHeterodermia obscurataHeterodermia jabonica3.Species found on the same sampling (Leeward) site but differ in substrateParmotrema tinctorum(tree/rock)4.Species restricted to the mid and high altitudesFlavoparmelia caperata5.Site-specific species (These are species found only in particular samplingCanoparmelia concrescenspoints and not seen in any other area)Cladonia sp, Sticta stenroosUsnea dasypoga,Usnea florida, Usnea articulate Sum of SquaresDf Mean SquareFSig.Between Groups17554.06928777.0354.321.022FluorineWithin Groups62966.666312031.183Total80520.73533Between Groups7630.71123815.3553.926.030ChlorineWithin Groups30129.76031971.928Total37760.47133Between Groups2814.60121407.3004.322.022BromineWithin Groups10093.42931325.594Total12908.02933 3Tukey HSDDependent VariableMean Difference (I-J)Std. ErrorSig.95% Confidence Interval Lower Upper Bound BoundFluorineLow (3-499m) Mid (500-1000m)51.849 *19.255.0304.4699.24High (>1000m)51.051 *20.257.0441.19100.91Mid (500-1000m) Low (3-499m)-51.849 *19.255.030-99.24-4.46High (>1000m)-.79918.159.999-45.4943.89High (>1000m)Low (3-499m)-51.051 *20.257.044-100.91-1.19Mid (500-1000m).79918.159.999-43.8945.49Chlorine Low (3-499m) Mid (500-1000m)36.357 *13.320.0273.5769.14High (>1000m)29.36414.012.107-5.1263.85Mid (500-1000m) Low (3-499m)-36.357 *13.320.027-69.14-3.57High (>1000m)-6.99412.561.844-37.9123.92High (>1000m)Low (3-499m)-29.36414.012.107-63.855.12Mid (500-1000m)6.99412.561.844-23.9237.91Bromine Low (3-499m) Mid (500-1000m)18.7627.709.053-.2137.74High (>1000m)22.333 *8.110.0262.3742.29Mid (500-1000m) Low (3-499m)-18.7627.709.053-37.74.21High (>1000m)3.5717.270.876-14.3221.46High (>1000m)Low (3-499m)-22.333 *8.110.026-42.29-2.37Mid (500-1000m)-3.5717.270.876-21.4614.32*. The mean difference is significant at the 0.05 level. 4Levene's Testfor Equality oft-test for Equality of MeansVariances95% ConfidenceFSig.TDfSig. (2-tailed)Mean DifferenceStd. Error DifferenceInterval of the DifferenceLowerUpperFluorineEqual3.568.068-.62332.538-16.53326.541-70.59537.528variancesassumedEqual-.4353.324.690-16.53338.030-131.15298.086variances notassumedChlorineEqual4.234.048-1.16932.251-20.93317.907-57.40815.541variancesassumedEqual-.7603.266.498-20.93327.552-104.71662.849variances notassumedBromineEqual2.837.102-.76132.452-8.06710.595-29.64813.515variancesassumedEqual-.5413.340.623-8.06714.914-52.90736.774variances notassumed Lichen Species as Bio-Accumulator of Some Halogens on Mount Cameroon Volcano, West Africa ## Acknowledgments We are grateful for the support by the University of Botswana Gaborone (UBG) where the chemical analyses were performed. We thank Professor Suh Tening Aaron of the University of Buea, who is part of the interdisciplinary research framework under the theme "Understanding the environment of Mount Cameroon". We thank Dr Smith B. Babiaka of the Department of Chemistry, University of Buea, for proof reading of the manuscript. ## Supplementary Information The online version of this article offers supplementary material (https://doi.org/xxxxx). ## Conflict of Interests The authors declare that there is no conflict of interest whatsoever Funding This study did not receive any funding. ## List of Tables * Use of bio-monitors to assess the atmospheric changes SAhmad MDaud IHQureshi Proceeding Pakistan Academy of Science 44 3 2007 * Volcanic plume monitoring at Mount Etna by diffusive (passive) sampling AAiuppa SBellomo AlessandroWFederico CFerm MValenza M J. Geophys. Res 109 2004 * Factors to consider for trace element deposition bio-monitoring surveys with lichen transplants SAyrault RClochiatti FCarrot LDaudin JBennett 10.1016/j.scitotenv.2006.10.032 The Science of the Total Environment 372 2007 * Determination of atmospheric heavy metals using two lichen species in Katni and Rewa cities RBajpai GKMishra SMohabe DKUpreti SNayaka India. J. Environ. Biol 32 2011 * Accumulation and toxic effect of arsenic and other heavy metals in a contaminated area of West Bengal, India, in the lichen Pyxine cocoes RBajpai DUpreti Sw.) Nyl. Ecotox. Environ 2012 * Safe 10.1016/j.ecoenv.2012.06.001 83 * Moss and Lichen Biomonitoring of Atmospheric Mercury: A Review RBargagli 10.1016/j.scitotenv.2016.07.202 Science of the Total Environment 572 31 2016 * Lichens and air pollution JPBennett International Society of Enviromental Botanists 12 4 2006 * Distribution patterns of usnic acid-producing lichens along local radiation gradients in West Greenland JWBjerke TDahl 10.1127/0029-5035/2002/0075-0487 Nova Hedwigia 75 2002 * An assessment of epiphytic lichen diversity and environmental quality in Knocksink Wood Nature Reserve LBrodeková AGilmer PDowding HFox GuttováA 10.3318/BIOE.2006.106.3.215 Proceedings of the Royal Irish Academy 106 2006 Biology * Lichens of North America IMBrodo SDSharnoff SSharnoff 2001 Yale University Press New Haven * Weathering of rocks induced by lichen colonization JChen HBlume LBeyer 10.1016/S0341-8162(99 Catena 39 2000 * Lichens as a tool for biogeochemical prospecting MKChettri TSawidis SKarataglis 10.1016/S0341-8162 Ecotoxicology and Environmental Safety 38 3 1997 * Trace elements deposition in the Tierra del Fuego region (south Patagonia) by using lichen transplants after the Puyehue-Cordón Caulle (north Patagonia) volcanic eruption in 2011. Environmental Science Pollution Research International MEConti RJasan MGFinoia IIavicoli RPlá 2016 23 * Environmental impacts on health from continuous volcanic activity at Yasur (Tanna) and Ambrym, Vanuatu SCronin DSharp Int. J. Environ. Health Res 12 123 2002 * Future directions in lichen chemistry CFCulberson WLCulberson 10.1639/0007-2745 The Bryologist 104 104 2001. 2001 0230: fdilc]2.0.CO * Biomonitoring by epiphytic lichen species Pyxine cocoes (Sw.) Nyl.: Understanding characteristics of trace metal in ambient air of different land uses in mid-Brahmaputra Valley RDaimari 10.1007/s10661-019-8007-x Environ. Monit. Assess 192 2020 * Historical Dictionary of the Republic of Cameroon MWDelancey DDMark 2000 3rd ed. * MarylandLanham The Scarecrow Press * Mount Cameroon: an active volcano of the Cameroon Line BDéruelle N'ni RKombou Journal of African Earth Science 6 2 1997 * Fluoride fact on human health and health problems: A review SDey BGiri Medical and Clinical Reviews 2 2 2015 * PS. LDiana VPatrícia SantosArmindo Dos 10.5772/intechopen.86058 2019 Environmental Health IntechOpen * Air Pollution and Air Quality NDonahue Green Chemistry: An Inclusive Approach Elsevier 2018 * Fluoride in the context of the environment MGGarcia LBorgnino Chemistry, Analysis, Function and Effects VRPreedy London The Royal Society of Chemistry 2015 * JGarty TLevin YCohen HLehr 2002 * Bio-accumulation behaviour of transplants of the lichen Flavoparmelia caperata in relation to total deposition at a polluted location in Portugal RMGodinho HTWolterbeek TGVerburg MCFreitas 10.1034/j.1399-3054.2002.1150213.x25 Environmental Pollution 115 2 2008 Physiologia Plantarum * Using of lichens for assessment of uranium atmosphere pollution AGolubev VGolubeva AKorableva NKrylov VKuznetsova SMavrin Aleinikov A Environment 3 36 2006 * Lichens as bioindicators in volcanic areas: Mt. Etna and Vulcano Island (Italy) MFGrasso RClocchiatti FCarrot Deschamps FVurro Environmental Geology 37 1999 * Epiphytic lichen diversity on dead and dying conifers under different levels of atmospheric pollution MHauck 10.1016/j.envpol.2004.09.021 Environmental Pollution 135 2005 * Production of oxalates in vitro by microbes isolated from rock surfaces with prehistoric paints in the Lower Pecos Region DHess DJCoker JMLoutsch Russ J 10.1002/gea.20208 Texas. Geoarchaeology 23 1 2008 * Diversity of lichens along altitudinal and land use gradients in the Trans Himalayan cold desert of Ladakh KJatinder KRoshni RHimanshu DKUpreti ATayade SHota OPChaurasia RBSrivastava 10.26438/ijsrbs/v6i1.97104 Nature and Science 10 4 2012 * The characteristics of rare earth elements in bulk precipitation, through fall, foliage and lichens in the Lesní potok catchment and its vicinity SJitka NTomás RJan MMartin KPetra PetraSLudek Geochemistry: Exploration, Environment 10 2010 Analysis * Lichen bioindication of biodiversity, air quality and climate: baseline results from monitoring in Washington SJovans Oregon and California. General Technical Report 2008 * Corticolous lichens as environmental indicators in urban areas in southern Brazil MIKäffer SM D AMartins CAlves VCPereira JFachel VM FVargas Ecological Indicators 11 5 2011 * Diversity of epiphytic lichens and their role in sequestration of atmospheric metals SKar ACSamal JPMaity SSantra 10.1007/s13762-013-0270-8 Int. J. Environ. Sci. Technol 11 2014 * Macroscopic changes of Hypogymnia physodes (L.) Nyl. in antropogenic stress conditions MJó?wiak Monit. ?rod. Przyr 13 2012 * Lichen and bryophyte distribution on oak in London in relation to air pollution and bark acidity RSLarsen JN BBell PWJames PJChimonides FJRumsey ATremper OWPurvis 10.1016/j.envpol.2006.03.033 Environmental Pollution 146 2007 * Biological role of lichen substances JDLawrey 10.2307/3242751 The Bryologist 89 1986 * Safety evaluation of fluoride content in tea infusions consumed in the Azores -a volcanic region with water springs naturally enriched in fluoride DLinhares PGarcia TFerreira Rodrigues Biological Trace Element Research 179 2017 * The use of lichen functional group asindicators of air quality in a mediterranean urban environment ELlop PPinho PMatos MJPereira CBranquinho 10.1016/j.ecolind.2011.06.005 Ecological Indicators 13 2012 * Epiphytic lichens as sentinels for heavy metal pollution at forest ecosystems (central Italy) SLoppi SAPirintsos 10.1016/S0269-7491(02 Environmental Pollution 121 2003 * Leaves of Pittosporum tobira as indicators of airborne trace element and PM10 distribution in central Italy GLorenzini CGrassi CNali APetti SLoppi LTognoti Atmospheric Environment 40 22 2006 * A new method to assess air pollution using lichens as bio-indicators JMonge-Nájera IGMaría RRMarta VHMéndez Revista de Biological Tropical 50 1 2002 * London: The Natural History Museum JMorris WPurvis 2007 19 Lichens (Life) * Physiological response of the epiphytic lichen Evernia prunastri (L.) Ach.to ecologically relevant nitrogen concentrations SMunzi LPaoli EFiorini SLoppi 10.1016/j.envpol.2012.07.001 Environmental Pollution 171 2012 * Moss and Lichen Biomonitoring of Atmospheric Pollution in the Western Cape Province (South Africa) NBNdlovu MVFrontasyeva RTNewman PMaleka 10.4236/ajac.2019.103008 American Journal of Analytical Chemistry 10 2019 * Persistence of epiphytic lichens along a tephra-depth gradient produced by the 2011 Puyehue-Cordón Caulle eruption in Parque Nacional PRNelson TBWheeler 2016 37 Puyehue, Chile. Bosque * Accumulation of volcanogenic fluoride by vegetation: Mt GNotcutt FDavies 10.1016/0377-0273(89 Journal of Volcanology and Geothermal Research 329 333 1989 * Lichen functional groups as ecological indicators of the effects of land-use in Mediterranean ecosystems PPinhoa ABergamini PCarvalhod CBranquinhoa SStofer SScheidegger CMáguas C 10.1016/j.ecolind.2011.09.022 Ecological Indicators 15 2012 * The use of epiphytic Antarctic lichens (Usnea aurantiacoatra and U. antarctica) to determine deposition patterns of heavy metals in the Shetland Islands APoblet SAndrade MScagliola CVodopivez ACurtosi APucci JMarcovecchio Antarctica. Science of the Total Environment 207 2-3 1997 * Periodic monitoring with lichen Phaeophyscia hispidula (ach.) Moberg in Dehradun city MRani VShukla DKUpreti GSRajwar The Environmentalist 31 4 2011 * Diffuse emission of organic trace gases from the flank and the crater of a quiescent active volcano FMSchwandner TMSeward APGize PAHall VDietrich J. Geophys.Res 109 D04301 2004 * Lichens to Biomonitor the Environment VShukla DKUpreti RBajpai 10.1007/978-81-322-1503-5 2014 * Investigation of trace element atmospheric pollution by nuclear analytical techniques at a global scale: Harmonised approaches supported by the IAEA BSmodis 10.1016/j.jenvman.2006.08.007 Journal of Environmental Management 85 2007 * Epiphytic lichen ramalina as indicator of atmospheric metal deposition, along land use gradients in and around binsar Wildlife Sanctuary KSrivastava PBhattacharya HRai PNag RKGupta 2015 Progress and Prosp Kumaun, Western Himalaya; India * Lichen Diversity and Lichen Transplants as Monitors of Air Pollution in a Rural Area of Central Italy LuisaStefano F 10.1007/s10661-006-4937-1 Environmental Monitoring and Assessment 114 2006 * Olivine-hosted glass inclusions from Scoriae erupted in 1954-2000 at Mount Cameroon volcano, West Africa CESuh JFLuhr MSNjome 10.1016/j.jvolgeores.2007.07.004 Journal of Volcanology and Geothermal Research 169 2008 * The 1999 and 2000 eruptions of Mount Cameroon: eruption behaviour and petrochemistry of lava CESuh RS JSparks JGFitton SNAyonghe CAnnen RNana ALuckman 10.1007/s00445-004-0388-0 Bulletin 65 2003 * Peroxyacetyl nitrates (PAN) and other minor pollutants. Recognition of Air Pollutant Injury to Vegetation: A Pictorial Atlas. 2nd Edition. Air and waste management association PJTemple JE JSun GH MKrause 1998 Pittsburgh * Long-term monitoring in the Netherlands suggests that lichens respond to global warming CMVan Herk AAptroot HFVan Dobben 10.1006/lich.2002.0378 Lichenologist 34 2 2002 * Regional variation in the chemical composition of winter snow pack and terricolous lichens in relation to sources of acid emissions in the Usa river basin, northeast European Russia TRWalker PDCrittenden SDYoung S0269-7491(03)00080-0 Environmental 125 2003 * Fluoride LHWeinstein ADavison UArndt Recognition of Air Pollutant Injury to Vegetation: A Pictorial Atlas. 2 nd Edition Pittsburgh, PA 1998 * WHO (2002) Fluorides. Geneva, World Health Organization Environmental Health Criteria 227 268